This research aimed to find the relationship between rejuvenating remedy and ICR mouse immune system. Screening of the methanolic extract of fifteen rejuvenating plants widely used in Thailand was conducted using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Among the plants studied, Butea superba Roxb, Plumbago indica L. and Miche/ia champaca L. were the three most active by MTT assay. They were further tested on lymphocyte proliferation with mitogen (lipopolysaccharide (LPS), pokeweed mitogen (PWM), phytohemagglutinin (PHA) and Concanavalin A (Con A)) as well as macrophage phagocytosis. The results showed that B. superba extracts (4 mg/ml) with LPS, PWM, PHA and Con A presented similar Stimulation Index (SI) value of about 1.3, suggesting moderate activation on both T- and B-lymphocyte proliferation. These observations revealed credible relation of rejuvenating remedy and the murine immune system.
Aurasorn Saraphanchotiwitthayaa, Kornkanok Ingkaninan and Pattana Sripalakit
Department of Pharmaceutical Technology, Faculty of Phannaceutical Sciences, Naresuan University, Phitsanulok 65000, Thailand.
Pharmaceutical Biotechnology Research Unit, Faculty of Phannaceutical Sciences, Naresuan University, Phitsanulok 65000, Thailand.
Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Science, Naresuan University, Phitsanulok 65000, Thailand.
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